UCSD Biochemical Genetics and Metabolomics Laboratory A Program in the Division of Genetics, Department of Pediatrics, University of California San Diego

Metabolomics

The UCSD Biochemical Genetics Laboratory

• Platform 1) Absolute quantification (targeted metabolomics) of >300 compounds including amino acids, organic acids, acylcarnitines, acylglycines, purines/pyrimides, acylcholines, and other classes are analyzed by triple quarupole LC-MS/MS (ABI SCIEX API 4000). 

  • Pricing is $300 per sample for two runs (positive and negative ionization modes). The cost is $150 if only one of the runs is desired, or $100 for any of the small sub-panels..

  • The targeted panels are listed here.
    For questions/ to plan sample referral, please contact Bruce Barshop and/or Jon Gangoiti.

• Platform 2) Discovery/untargeted metabolomics platform: Three runs on 2 different columns (multiple ionization modes) are performed using a high resolution/high mass accuracy Q-TOF mass spectrometer (AB SCIEX 5600), for comprehensive analysis of various polar, non-polar, and hydrophobic compound classes.

  • The analytes (several thousands) are processed by both univariate and multivariate statistical analysis, and statistically relevant analytes found to differentiate disease and control cohorts are identified and reported.

  • We use stable isotopes of over 50 compounds (amino acids, organic acids, acylcarnitines and purines/pyrimidines) along with standard curves and quality control samples run within the same batch, allowing for absolute quantification of many of these key metabolites (Method published: Gertsman et al. Metabolomics. (2014) 10:312-23).
    
    Pricing is $7000 per batch. A single batch includes up to 28 samples (recommended to have a minimum of 16, 8 of each cohort). Additional samples can be added to the batch at a cost of $300 per sample.  Please call or write to discuss your ideas and project goals with Dr. Ilya Gertsman in order to facilitate planning of a feasible project.