UCSD Biochemical Genetics and Metabolomics LaboratoryA Program in the Division of Genetics, Department of Pediatrics, University of California San Diego
The UCSD Biochemical Genetics Laboratory
Platform 1) Absolute quantification (targeted metabolomics) of >300 compounds including amino acids, organic acids, acylcarnitines, acylglycines, purines/pyrimides, acylcholines, and other classes are analyzed by triple quarupole LC-MS/MS (ABI SCIEX API 4000).
Pricing is $300 per sample for two runs (positive and negative ionization modes). The cost is $150 if only one of the runs is desired, or $100 for any of the small sub-panels..
Platform 2) Discovery/untargeted metabolomics platform: Three runs on 2 different columns (multiple ionization modes) are performed using a high resolution/high mass accuracy Q-TOF mass spectrometer (AB SCIEX 5600), for comprehensive analysis of various polar, non-polar, and hydrophobic compound classes.
The analytes (several thousands) are processed by both univariate and multivariate statistical analysis, and statistically relevant analytes found to differentiate disease and control cohorts are identified and reported.
use stable isotopes of over 50 compounds (amino
acids, organic acids, acylcarnitines and purines/pyrimidines)
along with standard curves and quality control samples run
within the same batch, allowing for absolute quantification of many
of these key metabolites (Method published:
Gertsman et al. Metabolomics. (2014) 10:312-23).
Pricing is $7000 per batch. A single batch includes up to 28 samples (recommended to have a minimum of 16, 8 of each cohort). Additional samples can be added to the batch at a cost of $300 per sample. Please call or write to discuss your ideas and project goals with Dr. Ilya Gertsman